兔胆总管部分结扎肝硬化模型的建立

Experimental Rabbit Model of Hepatic Cirrhosis Induced by Partial Bile Duct Obstruction

目的:探讨通过手术造成胆总管狭窄制备兔胆汁性肝硬化模型的可行性。方法:分离并部分结扎新西兰兔胆总管,结扎时分别与不同孔径的硬塑料管共同结扎或不用塑料管垫衬,结扎后将导管抽出,形成孔径分别为0、0.6mm、1.0mm和1.6mm的狭窄胆总管。存活兔于结扎14周后处死,观察其胆道系统变化情况。结扎前及结扎术后根据兔存活情况于1、2、4、11周经耳中央动脉抽血1.5mL测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)、总蛋白(TP)、白蛋白(ALB)。结果:兔胆总管部分结扎后有2种现象,一为肝内胆管形成球囊样扩张伴有胆石沉积,此类无明显肝纤维化表现;二为胆总管自狭窄以上呈不同程度扩张,有的呈现串珠样扩张,但无包裹性胆石沉积,此类肝纤维化程度较重。术后第1周,ALT、AST、TBIL、DBIL明显升高,第2周,ALT、AST、TP、ALB下降,TBIL、DBIL降至正常。结论:对新西兰兔进行胆总管部分结扎术可建立胆管阻塞型肝硬化模型。

Objective： To build up rabbit models of hepatic cirrhosis in through common bile duct partial ligation （CBDPL）. Methods： Male New Zealand rabbits were subjected to CBDPL to induce hepatic cirrhosis. The liver biopsies were performed during the surgery and after sacrifice to evaluate hepatic fibrosis. The serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, total bilirubin （TBIL）, total protein （TP） and albumin （ALB） were determined at week 0, 1, 2, 4 and 11 following the occlusion. Results： There were two kinds of morphological and histological manifestations after CBDPL, the one was inconspicuous hepatic fibrosis coexisted with sacculation-like bile ductal dilation and muddy stone; the other is remarkable hepatic fibrosis and cirrhosis along with uneven bile ductal dilation. The serum levels of ALT, AST, TBIL and DBIL were significantly higher after one week of operation. The levels of ALT, AST, TP and ALB decreased after two-week of operation, and the levels of TBIL and DBIL were returned to the normal level after two-week of operation. Conclusion： The animal models of hepatic cirrhosis can be built up through CBDPL in rabbits.