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结肠癌组织NRP2的表达与淋巴结转移的关系 被引量：9

The relationship of neuropilin-2 expression with lymphangiogenesis in colorectal carcinoma
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摘要 目的:探讨结肠癌组织中NRP2的表达在淋巴管生成和转移中的作用。方法:采用免疫组化法、RT-PCR法检测结肠癌组织中NRP2和VEGF-C的表达,以及结肠癌组织淋巴管的免疫组化标记和微淋巴管密度(MLD)计数。结果:结肠癌组织周围区域(48.8±23.5)、表浅区域(24.6±10.1)和肿瘤中心(11.3±7.7)MLD存在显著差异(P<0.05);结肠癌组织NRP2的mRNA水平为0.87±0.26,高于正常组织0.38±0.12,转移组的mRNA水平为1.12±0.25,也高于非转移组的0.76±0.19(P<0.01)。NRP2表达定位于结肠癌细胞浆和部分淋巴管内皮细胞浆,阳性率为34.8%;其表达与肿瘤边缘及标记部位MLD呈正相关(r=0.432,P<0.01),并与肿瘤的淋巴结转移、分化程度、Dukes分期和浸润深度相关(P<0.05),与VEGF-C的表达亦呈正相关(r=0.606,P=0.001)。结论:NRP2表达可能对结肠癌局部淋巴管生成及淋巴结转移起到重要的作用。 Objective：To explore the role of neuropilin-2 （NRP2） expression in coloreetal cancer tissue lymphangiogenesis and metastasis. Methods：Detected the expression of NRP2, and VEGF-C with immunohistoehemical and reverse transcription-poly- merase chain reaction （RT-PCR） analysis, furthermore, detected the micro-lymphantic density （MLD） with immunohistoehemieal anal- ysis. The results were analyzed statistically to investigate the correlations of NRP2 and VEGF-C expression with lymphonode metastasis, and MLD, and some other clinical pathology charactericties. Results：The significant differences of MLD were among those of tumor margin（48. 8 ±23.5 ） , superficial tumor region（24. 6± 10. 1 ） and tumor center（ 11.3 ±7.7） （P 〈0.05）. The mRNA level of NRP2 in tumor tissue（0. 87± 0. 26） was higher than that（0. 38 ± 0. 12） in normal tissue （P 〈 0. 01 ）. The mRNA level of NRP2 （ 1.12 ±0. 25 ） in tumor tissue of metastasis group was higher than that （0. 76± 0. 19 ） in non-metastasis group （ P 〈 0. 01 ）. The expression of NRP2 were location on cytoplasm in tumor cells and some lymphantie endothelial cells（ LECs）, it＇s expression rate was 34. 8%. Their MLD were correlated with expression positive strength of NRP2 （ r = 0. 432, P 〈 0. 01 ）. The expression of NRP2 was association with lymphatic node metastasis, and differentiation degree, and Dukes stage and infiltrative degree of tumors（P 〈0.05）. The expression of NRP2 in colorectal cancer tissue was positively correlated with expression of VEGF-C （ r = 0. 606, P = 0. 001 ）. Conclusion . The expres- sion of NRP2 in colorectal cancer tissue is associated with lymphangiogenesis and lymph nodes metastasis in colorectal carcinoma.
作者 周琪 梁后杰 阎晓初 彭秋平 周进明 吴峰 龙艺 刘以淑
机构地区 重庆市涪陵中心医院肿瘤科 第三军医大学西南医院肿瘤科 第三军医大学西南医院病理研究所
出处 《临床肿瘤学杂志》 CAS 2009年第9期797-802,共6页 Chinese Clinical Oncology
基金 国家自然科学基金资助项目(30572159)
关键词 NRP2 VEGF-C 淋巴管生成 转移 NRP2 VEGF-C Lymphangiogenesis Metastasis
分类号 R735.3 [医药卫生—肿瘤]
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参考文献15

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二级参考文献28

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共引文献42

同被引文献151

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引证文献9

二级引证文献37

临床肿瘤学杂志
2009年 第9期

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