NF-κB、c-myc和survivin在非霍奇金淋巴瘤中的表达及临床意义

Expressions of NF-κB,c-myc and survivin in non-Hodgkin's lymphoma and their clinical significance

目的:探讨非霍奇金淋巴瘤(non-Hodgkin’s lymphoma,NHL)中NF-κB、c-myc和survivin蛋白表达水平及其临床意义。方法:采用免疫组织化学PV-9000二步法检测62例NHL肿瘤组织及18例淋巴结反应性增生(reactive hyperplasia oflymph node,RH)组织中NF-κB、c-myc和survivin的表达情况,并分析蛋白表达与临床病理特征、化疗疗效及预后的相关性。结果:NF-κB、c-myc和survivin蛋白在NHL组织中的阳性表达率分别为61.29%、77.42%和72.58%,高于RH组织中的表达率16.67%、22.22%和11.11%(P<0.01)。NF-κB、c-myc和survivin的表达与NHL的恶性程度、临床分期均呈正相关(P<0.05),而与患者的年龄、全身症状、PS评分和淋巴结外病变数目均无相关性(P>0.05),其中NF-κB、c-myc在T细胞淋巴瘤中的阳性表达率高于B细胞淋巴瘤(P<0.05)。NF-κB、c-myc和survivin在NHL组织中阳性表达率与其化疗疗效呈负相关(r=-0.362、r=-0.296和r=-0.273,P<0.05)。NHL组织中NF-κB、c-myc和survivin表达之间均存在正相关关系(r=0.514、r=0.476和r=0.313,P<0.05),且NF-κB和c-myc在国际预后指数(international prognostic index,IPI)高危组中的表达均高于IPI低危组(P<0.05)。结论:NF-κB、c-myc和survivin的表达状况与NHL患者恶性程度、临床分期及化疗疗效存在相关性,可作为评价NHL患者预后的生物学参考指标。

Objective：To study the expression levels of NF-κB,c-myc and survivin in non-Hodgkin＇s lymphoma（NHL） and their correlation with the clinicopathological characteristics,clinical outcome,and prognosis of patients with NHL.Methods：The expression levels of NF-κB,c-myc,and survivin protein were evaluated in the tissues of 62 patients with NHL as well as 18 reactive hyperplasia of lymphnode（RH） by immunohistochemistry（PV-9000 method）.Results：The positive expression rates of NF-κB,c-myc,and survivin were 61.29%,77.42%,and 72.58% in NHL tissues,higher than that in RH tissues（16.67%,22.22%,and 11.11%,P〈0.05）.Expressions of NF-κB,c-myc,and survivin protein had a positive correlation with malignant degree and clinical staging（P〈0.05）,but had no correlation with the age,whole body symptom,PS score,and the number of extranodal lesions（P〉0.05）.The protein expressions of NF-κB,c-myc,and survivin in T-lympocyte lymphoma were higher than that in B-lympocyte lymphoma（P〈0.05）.There was a negaitive correlation between the expressions of NF-κB,c-myc,and survivin protein in NHL tissues and therapeutic times（r=-0.362,r=-0.296,and r=-0.273,P〈0.05）,and there were positive correlations among the expressions of NF-κB,c-myc,and survivin protein in NHL（r=0.514,r=0.476,and r=0.313,P〈0.05）.Expressions of NF-κB and c-myc in international prognostic index（IPI） high-risk group of NHL were higher than those in IPI low-risk group（P〈0.05）.Conclusion：There is a significant correlation between the expressions of NF-κB,c-myc,and survivin in NHL and malignant degree,clinical sta-ging,and chemotherapeutic efficacy.Expressions of NF-κB,c-myc and survivin protein could be used as a reference index for predicting the prognosis of NHL.