MKK4基因在喉鳞癌中的表达及其临床意义

Expression of MKK4 gene in laryngeal squamous cell carcinoma and its clinicopathological significance

目的:研究丝裂原活化蛋白激酶激酶4(mitogen-activated protein kinase kinase 4,MKK4)在喉鳞状细胞癌组织中的表达及其临床意义。方法:应用RT-PCR和免疫组织化学方法检测42例喉鳞癌、20例喉乳头状瘤及20例声带息肉组织中MKK4 mRNA和蛋白的表达,分析其与喉鳞癌临床病理参数之间的关系。结果:MKK4 mRNA和蛋白表达的阳性率在喉鳞癌、喉乳头状瘤及声带息肉组织中逐渐降低,且差异有统计学意义(P<0.05)。MKK4 mRNA和蛋白的表达随喉鳞癌的浸润深度增加而上升(P<0.05),在有淋巴结转移组中的表达高于无淋巴结转移组(P<0.05),在临床Ⅲ～Ⅳ期患者中的表达高于临床Ⅰ～Ⅱ期的患者(P<0.05)。MKK4的表达与患者的年龄、性别、肿瘤发生部位及组织学分级无相关性(P>0.05)。结论:MKK4 mRNA和蛋白在喉鳞癌组织中的表达上调可能与喉鳞癌的发生及浸润转移有关,MKK4可能成为一个潜在的判断喉鳞癌发生与转移的观测指标。

Objective：To investigate the expressions of mitogen-activated protein kinase kinase 4（MKK4） mRNA and protein in laryngeal squamous cell carcinoma（LSCC） and its clinical significance.Methods：Reverse transcriptase polymerase chain reaction（RT-PCR） and immunohistochemical methods were used to examine the expression of MKK4 in 42 cases of LSCC,20 cases of laryngeal papilloma（LP）,and 20 cases of vocal cord polyp（VCP）.The relationship between MKK4 expression and the clinicopathological parameters of LSCC was analyzed.Results：MKK4 mRNA and protein expressions were gradually decreased from LSCC to LP and VCP.The difference was significant（P〈0.05）.MKK4 mRNA and protein expressions were increased with the increase in the invasive depth of LSCC（P〈0.05）.MKK4 mRNA and protein expressions were significantly higher in LSCC with nodal metastasis and at Ⅲ to Ⅳ cli-nical stage than those without nodal metastasis and at Ⅰto Ⅱ clinical stage（P〈0.05）.The positive expression of MKK4 was not rela-ted with the age,gender,primary site,and histologial classification of LSCC patients（P〉0.05）.Conclusion：The up-regulation of MKK4 mRNA may be related with the initiation,invasion and metastasis of LSCC.MKK4 may serve as a valuable marker for evaluating the carcinogenesis and metastasis of LSCC.