姜黄素对人结肠癌细胞SW480作用的体外实验研究

The effect of Curcumin on human colon cancer cells SW480 in vitro

目的研究姜黄素促进人结肠癌细胞系SW 480凋亡的作用。方法人结肠癌细胞系SW 480与不同浓度的姜黄素共孵育48 h,MTT法检测细胞存活率,并用相差显微镜观察细胞形态学改变。用流式细胞术(检测细胞凋亡相关指标Annexin V/PI)和Ho-echst33258染色检测细胞凋亡。结果姜黄素能够不同程度地抑制人结肠癌细胞系SW 480的生长。姜黄素对SW 480细胞的IC50(半数抑制浓度)为65 mg/mL。Hoechst33258荧光染色与流式细胞术分析SW 480细胞的结果一致。结论姜黄素可促进SW 480细胞凋亡,为姜黄素治疗结肠癌提供依据。

Objective To investigate the effect of Curcumin accelerating the apoptosis of human colon cancer cell line SW480 in vitro.Methods Human colon cancer cell line SW480 was incubated with different concentrations of Curcumin for 48 h,then cell survival rate was measured by MTT assay and cell morphology change was observed by Phase contrast microscope.Apoptosis was detected by flow cytometry（Annexin V/PI staining） analysis and Hoechst 33258 staining.Results Curcumin could inhibit the growth of SW480 in varying degrees.The IC50 value of Curcumin in SW480 cells was 65 mg/mL.The number of apoptotic SW480 cells calculated with flow cytometry was accordant with the result with Hoechst 33258 staining.Conclusion Curcumin can promote apoptosis SW480 cells.It provides the evidence for Curcumin treating colon cancer.