辣椒胞质雄性不育系与保持系蕾期基因表达差异分析

Differential Expression Analysis in Flower Buds of CMS and Its Maintainer Line of Capsicum annuum L.

利用cDNA-AFLP差显技术比较分析了辣椒细胞质雄性不育系及其保持系蕾期基因的表达差异,获得了19条阳性差异转录片段,其中在不育系花蕾中特异表达的有4条,在保持系花蕾中特异表达的有15条。通过对19条差异片段进行序列同源性分析与功能分类,结果表明:在不育系花蕾中特异表达的其中3条与番茄和烟草线粒体、核糖体代谢相关基因有很高的相似性;在保持系花蕾中特异表达的12条能找到与其相似性较高的序列,这些差异片段代表的基因涉及转录调控、防卫和胁迫反应、电子传递和能量途径、DNA或RNA代谢、蛋白质代谢、转运通道、未知或假定蛋白等。推测辣椒细胞质雄性不育的发生可能受到多种代谢途径的共同调控。

In order to understand the mechanism of CMS in hot pepper, the gene expression in flower buds of CMS line and its maintainer line was compared by means of cDNA-AFLP. A total of 19 differential transcript-derived fragments （TDFs） were identified and sequenced. Four TDFs were expressed specifically in CMS line, whereas 15 others could be detected only in the maintainer line. The analysis of sequence homology and functional classification indicated that three TDFs coming from CMS line shared high identities with mitochondrial or ribosomal RNA genes of tomato and tobacco. 12 TDFs from maintainer line were highly homologous to known sequences. These genes might be related to transcription, defense response, electron transport and energy pathway, DNA or RNA-mediated metabolism, protein metabolism, transport, unknown or putative protein and so on. It is speculated that the occurrence of CMS in pepper might involve different metabolism pathways.