基于原子力显微镜测量内耳螺旋器的弹性特征

LOCAL ELASTIC PROPERTIES OF THE GUINEA PIG ORGAN OF CORTI MEASURED BY ATOMIC FORCE MICROSCOPY

应用原子力显微镜分析内耳螺旋器(Corti器)不同部位的弹性特征。采用豚鼠内耳基底膜底回新鲜标本,用原子力显微镜在液相接触式测量,获得不同部位力曲线。经计算,对应Corti器相当于Hensen细胞、外毛细胞、柱细胞、内毛细胞、内指细胞、盖膜的部位及基底膜底面局部,其杨氏模量均值分别为46±1.7、59±0.9、250±31、140±2.8、430±29.9、210±7.2和230±8.8kPa。结果表明,基底膜径向排列的组织结构不同,杨氏模量存在明显差异,在整块基底膜标本上测量Corti器各结构的杨氏模量能更准确地反映它们在生理状态下的弹性特征。

The elastic properties of the guinea pig organ of Corti were investigated using atomic force microscopy in this study for mechanical modeling of the organ of Corti. The fresh basilar membranes of guinea pig were measured by the contact method using atomic force microscopy under aqueous environment, and the force curves of the different cells in the organ of Corti were acquired. The Young＇s modulus was calculated to be （46±1.7）kPa for Hensen＇s cells, （59±0.9）kPa for outer hair cells, （250±31） kPa for pillar cells, （140±2.8）kPa for inner hair cells, （430±29.9）kPa for inner phalangeal cells, （210±7.2） for tectorial membrane and （230±8.8）kPa for basilar membrane, respectively. It is concluded that the different cells in the organ of Corti under physiological environment have different elastic properties. The measured Young＇s modules of the cells on the intact organ of Corti reflects their real elastic properties more precisely than that of detached cells of the organ of Corti.