摘要
目的研究普伐他汀对载脂蛋白(apo)E-/-小鼠血脂、肿瘤坏死因子(TNF)α及动脉粥样硬化血管B细胞淋巴瘤(Bcl-2)及相关X蛋白(Bax)表达的影响。方法应用高胆固醇饲料建立apoE-/-小鼠动脉粥样硬化模型,采用普伐他汀混悬液灌胃,实验8周后,酶联免疫吸附法及反转录聚合酶链式扩增法测定血清及主动脉TNFa水平,并应用Western blot法检测主动脉Bax及Bcl-2蛋白的表达,苏木素伊红(HE)染色观察主动脉组织形态学变化。结果普伐他汀可降低动脉粥样硬化apoE-/-小鼠血清及动脉TNFα水平(34.8±22.7)μg/L与(180.1±59.1)μg/L,改善主动脉组织病理损伤,调控动脉粥样硬化血管Bax及Bcl-2蛋白表达。结论普伐他汀可降低血脂,干预动脉粥样硬化的始动环节,并从蛋白水平调控动脉粥样硬化内皮细胞凋亡。
Objective To investigate the effects of pravastatin on the serum lipids, the tumor necrosis factor (TNF)α and the expressions of Bax and Bcl 2 in apoE-/- mouse. Methods The atherosclerosis model of apoE-/- mouse was induced by high cholesterol diet; and the suspension of pravastatin was administered intragastrically. After 8 weeks, the levels of serum TNFα and mRNA of aortic TNFα were detected by enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR), respectively. The expressions of Bax and Bcl-2 were tested by Western blot analysis and aortic histomorphology changes was observed by Hematoxylin-Eosin (HE) stainning. Results In atherosclerosis model of apoE-/- mouse, the serum lipids, the levels of serum and aortic TNFα[(34. 8±22. 7) μg/L and(180. 1 ±59. 1 )μg/L], the expressions of Bax and Bcl-2 and aortic histomorphology were obviously improved by pravastatin. Conclusions It is suggested that the pravastatin can reduce serum lipid, intervene the initiation step of atherosclerosis and regulate endothelial cells apoptosis.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2009年第9期765-768,共4页
Chinese Journal of Geriatrics