普伐他汀对动脉硬化鼠肿瘤坏死因子α和B淋巴瘤-2及相关X蛋白的影响

The effects of pravastatin on expressions of tumor necrosis factor α,Bax and Bcl-2 in apoE-/- mouse

目的研究普伐他汀对载脂蛋白（apo）E-／-小鼠血脂、肿瘤坏死因子（TNF）α及动脉粥样硬化血管B细胞淋巴瘤（Bcl-2）及相关X蛋白（Bax）表达的影响。方法应用高胆固醇饲料建立apoE-/-小鼠动脉粥样硬化模型，采用普伐他汀混悬液灌胃，实验8周后，酶联免疫吸附法及反转录聚合酶链式扩增法测定血清及主动脉TNFa水平，并应用Western blot法检测主动脉Bax及Bcl-2蛋白的表达，苏木素伊红（HE）染色观察主动脉组织形态学变化。结果普伐他汀可降低动脉粥样硬化apoE-／-小鼠血清及动脉TNFα水平（34．8±22．7）μg／L与（180．1±59．1）μg／L，改善主动脉组织病理损伤，调控动脉粥样硬化血管Bax及Bcl-2蛋白表达。结论普伐他汀可降低血脂，干预动脉粥样硬化的始动环节，并从蛋白水平调控动脉粥样硬化内皮细胞凋亡。

Objective To investigate the effects of pravastatin on the serum lipids, the tumor necrosis factor （TNF）α and the expressions of Bax and Bcl 2 in apoE-/- mouse. Methods The atherosclerosis model of apoE-/- mouse was induced by high cholesterol diet; and the suspension of pravastatin was administered intragastrically. After 8 weeks, the levels of serum TNFα and mRNA of aortic TNFα were detected by enzyme linked immunosorbent assay （ELISA） and reverse transcription polymerase chain reaction （RT-PCR）, respectively. The expressions of Bax and Bcl-2 were tested by Western blot analysis and aortic histomorphology changes was observed by Hematoxylin-Eosin （HE） stainning. Results In atherosclerosis model of apoE-/- mouse, the serum lipids, the levels of serum and aortic TNFα[（34. 8±22. 7） μg/L and（180. 1 ±59. 1 ）μg/L], the expressions of Bax and Bcl-2 and aortic histomorphology were obviously improved by pravastatin. Conclusions It is suggested that the pravastatin can reduce serum lipid, intervene the initiation step of atherosclerosis and regulate endothelial cells apoptosis.