CCR5Δ32等位基因多态性与贵州特殊人群HBV感染的相关性

Association of CCR5-Δ32 polymorphism with HBV infection in special population of Guizhou Province

目的:探讨CCR5Δ32等位基因在贵州世居少数民族(彝、瑶)与汉族人群的分布,并分析CCR5位点突变与HBV的关系.方法:采用PCR技术分别扩增贵州92份黔西彝族、101份威宁彝族、138份荔波瑶族以及165份毕节汉族CCR5编码区片段,通过琼脂糖凝胶电泳对CCR5Δ32多态性进行分析,最后抽取样本并进行DNA测序验证.结果:经过PCR特异性扩增,琼脂糖凝胶电泳后,496份样本均未发现CCR5Δ32突变型和杂合子.DNA测序验证所有样本CCR5基因未发生32碱基缺失的突变.结论:CCR5Δ32的分布有较明显的地域和种族差异,该位点与HBV感染的相关性有待于进一步深入研究.

AIM： To investigate the distribution of CCR5-△32 polymorphism in Han nationality, Yi and Yao minorities of Guizhou and analyze the association between CCR5-△32 polymorphism and HBV infection. METHODS： Blood samples were taken from 92 Chinese Yi ethic individuals in Qianxi, 101 Chinese Yi ethic individuals in Weining, 138 Chinese Yao ethic individuals in Libo and 165 Chinese Han ethic individuals in Bijie. The coding region of the CCR5 gene was amplified by PCR and resolved by agarose gel electrophoresis to analyze the polymorphism of CCR5-△32. DNA sequencing was performed to verify the above results. RESULTS： The 32-base pair deletion allele in the CCR5 gene was not found in all 496 samples. DNA sequencing verified that no CCR5-△32 allele was detected in all samples. CONCLUSION： No CCR5-△32 allele is detected in this study in special population of Guizhou Province though the distribution of CCR5-△32 allele has geographical and ethnic differences.