急性髓系白血病的免疫分型特点

The Characteristics of Immunophenotype in Acute Myeloid Leukemia

目的:探讨急性髓系白血病(AML)免疫分型的特点。方法:采用流式细胞术对208例AML患者进行白血病免疫分型检测。结果:①表达率最高的AML抗原为CD33(99.5%)、CD117(98.6%)、CD123(97.6%)、CD13(92.3%)及MPO(90.9%)。②伴淋系抗原表达者以CD7(47.1%)最高,其次为:CD2(13.9%)、CD4(13.5%)及CD19(4.3%)。③AML微分化型(M0)和急性巨核细胞白血病(M7)表达2个以上的髓系抗原,AML其他各型均能表达3个以上的髓系抗原;CD19仅表达于急性粒细胞白血病部分分化型M2b型(M2b);CD2和CD9仅表达于急性早幼粒细胞白血病(M3);CD4和CD14仅表达于急性粒单核细胞白血病(M4)和急性单核细胞白血病(M5)。结论:①CD33、CD117、CD123、CD13及MPO为髓系最敏感的抗原。②CD2、CD4、CD9、CD14及CD19在AML诊断上具有重要意义。

Objective： To investigate the characteristics of immunophenotype in acute myeloid leukemia（AML）. Method： Immunophenotyping was performed by flow cytometry in 208 patients with AML. Results： The incidence of myeloid antigen expression was 99.5% of CD33, 98.6% of CD117, 97.6% of CD123, 92.3% of CD13 and 90.9% of MPO respectively. The highest incidence of accompanying lymphoid antigen expression was 47.1% of CD7, 13.9% of CD2, 13.5% of CD4 and 4.3% of CD19 respectively. Two or more myeloid associated antigens were expressed on M0 and M7 subtypes of AML, three or more myeloid associated antigens were expressed on other subtypes of AML. CD19 was expressed only on M2b subtype, CD2 and CD9 were expressed on M3 subtype, while CD4 and CD14 were expressed on M4 and M5 subtype respectively. Conclusion： （1）CD33, CD117, CD123, CD13 and MPO are sensitive myeloid associated antigens. （2）The expression of CD2, CD4, CDg, CD14 and CD19 has important significance in AML diagnosis.