人前脂肪细胞株HPA-v的培养及诱导分化

Culture and Differentiation of Human Preadipocytes HPA-v

为建立稳定的人源前脂肪细胞株体外诱导分化模型,作者研究了人源原代前脂肪细胞株HPA-v的生长及分化过程.研究结果表明HPA-v细胞的生长潜伏期为2天,第3天细胞进入对数生长期,第5天细胞生长停滞.显微镜观察、油红O染色、GPDH活性检测结果表明第3天细胞分化特征开始出现,细胞内脂滴含量开始增加,GPDH活性开始升高,12天左右基本分化为成熟的脂肪细胞,脂滴含量及GPDH活性达到最高峰.

In order to develop stable differentiation model of preadiocytes, this paper studied the culture and differentiation of HPA-v （human preadiocytes）.The results show that the latent period was two days, log phase occurred at the 3rd day and Stationary phase the 5th. The differentiation process was detected by inverted microscope, Oil Red O staining and GPDH activity measurement. The results showed that the differentiation appeared at the 3rd day, the intracytoplasmic lipids increased along with the differentiation time, and GPDH activity shared the same regularity which increased quickly at the 3rd day. The maximum value of intracytoplasmic lipids and GPDH activity was achieved at the 12th day and maintained high level.