摘要
利用分光光度计对里氏木霉重组t-PA的性质进行了研究,结果表明该酶对高温较为敏感,60℃酶完全失活;在pH6.4-7.6范围内,作用时间的长短对酶活力影响不大,保持较大的酶活;Zn^2＋和Fe^3＋离子对其有抑制的作用,Mg^2＋、Ca^2＋、Cu^2＋、Fe^2＋、Mn^2＋、Na^＋、K^＋对酶有激活的作用。
The properties of Trichoderma reesei -reorganizing t -PA were studied with spectrophotometer. The results showed that this enzyme was more sensitive at high temperature, and completely inactivated at 60℃. Reaction time had little influence on enzyme activity at the range of pH from 6.4 to 7.6, Zn^2＋ and Fe^3＋ had inhibitory effect on the activity, and Mg^2＋ , Ca^2＋ , Cu^2＋ , Fe^2＋ , Mn^2＋ , N^a＋ and K^＋ could activate the enzyme.
出处
《氨基酸和生物资源》
CAS
2009年第3期33-35,共3页
Amino Acids & Biotic Resources
关键词
里氏木霉
T-PA
性质
Trichoderma reesei
t - PA
property
