基因组水平上杨树NAC基因家族的初步分析

Primary Genome-wide Analysis of the NAC Gene Family in Poplars

利用其他物种的NAC蛋白,构建NAC结构域的位置特异得分矩阵(PSSM).用PSSM搜索整个杨树PopulusL.基因组,获得编码杨树NAC结构域的DNA片段在基因组上的位置信息,然后运用Perl脚本语言从基因组相应位置提取包含基因全长的序列,推测NAC基因的全长、氨基酸序列及其结构特征,并对得到的杨树NAC基因家族进行了系谱发生分析.结果表明:杨树中至少存在132个非冗余的NAC基因,系谱分析表明这些NAC基因归属于14个亚家族,同一亚家族内各基因编码NAC结构域的外显子数量和结合位置的分布具有一定的相似性.

The gene family of NAC （NAM, ATAF1, and CUC2） is involved in plant developmental processes, and in plant hormonal control and defense. The amino acid sequences of NAC are characteristic of a highly conserved N-terminal NAC domain. This study created a position-specific scoring matrix （PSSM） of NAC domain with all the identified NAC proteins from other species and identified 132 NAC genes in poplars （Populus L. ） by searching the genome with PSSM. An overview of these genes is presented, including the coordinates of gene in chromosomes, location of the NAC domain in a gene, and exon-exon junction positions. A genealogical investigation of this gene family shows that its 132 NAC genes are classified into 14 subfamilies, within each of which a similar number of exons and a similar distribution of exon-exon junction in each NAC domain are conserved.