摘要
目的通过改进大鼠心肌缺血/再灌注模型制备方法,提高造模成功率。方法雌雄各半Wist-ar大鼠40只,随机分为伪手术(S)组20只和心肌缺血再灌注(I/R)组20只。整个实验过程在人工机械通气下完成,所有操作尽量不破坏动物组织结构。结果I/R组结扎心肌左冠状动脉前室间支后心电图ST段弓背向上抬高0.4 mV以上,S组手术前后无变化;I/R组造模成功率为90%。结论本方法制备大鼠心肌缺血/再灌注模型简便易行、心脏暴露好、结扎可靠、结果稳定。
Objective To improve the method of establishing in vivo myocardial ischemic-reperfusion rat model and to increase the success rate. Methods Forty （20 male, 20 female） Wistar rats were randomly divided into Sham group （n= 20） and myocardial ischemic-reperfusion group （I/R, n= 20）. In I/R group , coronary occlusion for 45 rain was followed by 3 hours reperfusion of left coronary artery. In the period of experiments, rats breathed under the control of artificial respirator. Results The 90 percent of total rats were made myocardial isehemia-reperfusion model successfully. In I/R group., the ST segment elevated more than 0.4 mV. Conclusion The method is simple, convenient and the experiment results are reliable.
基金
山西省人民医院青年科研资助项目(200825)
关键词
心肌再灌注
模型
动物
大鼠
Myocardial reperfusion
Models, animal
Rats
