GHRP温控缓释系统的建立及对动物生长的影响

The establishment of GHRP thermo-sensitive delivery system and its influence on animal growth

在体凝胶（In situgel）在药学、生物技术领域各个方面的应用，包括药物输送、细胞膜包覆和组织修复等。本试验采用了温敏缓释载药系统，制备了GHRP-6温敏缓释系统，研究其对动物生长的影响。结果显示，Vc系獭兔肌注GHRP-6温敏缓释凝胶7、14、21、28d后，2、4g／L组累积增重效果分别比生理盐水组高25．83％～63．37％；肌注GHRP-6温敏缓释凝胶血清IGF—Ⅰ浓度：注射7d后，2、4g／L组分别比生理盐水组高16．31％（P〈0．05），27．98％（P〈0．01）；注射14d后，2、4g／L组分别比生理盐水组高37．43％（P〈0．01）、47．17％（P〈0．01）；注射28d后，2、4g／L组分别比生理盐水组高31．71％（P〈0．01）、40．14％（P〈0．01）。试验证明，GHRP-6温敏缓释凝胶能显著增高血清中IGF—Ⅰ浓度，促进Vc系獭兔生长，血清中IGF—Ⅰ浓度与Vc系獭兔累积增重呈正相关。

In recent years, there are many reports on the gel （In situgel） application in the pharmaceutics and biotechnology,including drug delivery,membrane coating and tissue repairing. In this research,we established the GH- RP-6 of the thermo-sensitive drug delivery system and studied its effect on animal growth. At 7 d, 14 d, 21 d, 28 d after Vc Rex rabbits were intramuscularly injected with GHRP-6 thermo-sensitive delivery At the cumulative weight gain in 2 g/L group and 4 g/L group was higher by 25.83%-63. 37% than that of saline group. In addition,Vc Rex rabbits were intramuscularly injected with GI-IRP-6 thermo-sensitive delivery of gel serum IGF-Ⅰ concentrations. Compared with that of normal saline group, the serum IGF-Ⅰ concentrations in 2 g/L group and 4 g/L group was higher by 16.31% （P〈0.01） and 27.98% （P〈0.01） respectively at 7 d after injection;37.43% （P〈0.01） and 47.17% （P〈0.01）at 14 d after injection;31.71% （P〈0.01）and 40.14% （P〈0.01l） at 28 d after injection. These results indicated that the injection with GHRP-6 thermo-sensitive delivery increased 1GF-I concentration in the serum significantly and promoted the cumulative weight gain. The cumulative weight gain was positively correlated with IGF-Ⅰ concentration in the serum.