体外受精-胚胎移植后过夜培养精子活率与受精率关系的探讨

Relationship between human 24-h sperm vitality in vitro and fertilization rate

目的探讨过夜培养后精子活率与体外受精（IVF）受精率的关系。方法回顾性分析2007年1—12月北京大学第三医院811个常规IVF受精后精子过夜培养后的活率。分为过夜精子活率〈20％组（A组），过夜精子活率20％～80％组（B组），过夜精子活率〉80％组（C组），比较三组的受精率，可用胚胎率。结果A组受精率（47．6％）或B组受精率（57．2％）明显低于C组受精率（62．7％），差异具有统计学意义（P〈0．05），过夜精子活率〈80％组（A组＋B组）的可用胚胎率（72．1％）低于活率〉80％组（77．4％），两者比较差异有统计学意义（P〈0．05）。结论精子过夜培养对受精率的评价有一定的参考价值和意义。

Objective To study on the relationship between human 24-h sperm vitality in-vitro and fertilization rate. Methods 811 in-vitro fertilization （IVF） cycles were divided into three groups, 24-h sperm vitality 〈 20% （group A）, 24-h sperm vitality 20% - 80% （ group B） and 24-h sperm vitality 〉 80% （ group C） respectively. All samples of semen were cultured for 24 hours in vitro and examined the vitality. Fertilization rate, good-quality embryo rate were compared among the three groups. Results The fertilization rate in group A （47.6%） or group B （57. 2% ） were significantly lower than those in group C（ 62. 7% ）, P 〈 0.05. The good-quality embryo rate in vitality 〈 80% group（ A ＋ B） was significantly lower than that in vitality 〉 80% group （72. 1% vs77. 4% ）, P 〈 0. 05. Conclusion 24-h sperm vitality can be regarded as a predictive index of fertilization rate.