摘要
目的:通过检测神经干细胞移植后Wistar大鼠脑缺血再灌注损伤后局部STAT3蛋白和bcl-2蛋白表达水平的变化,来研究移植入的神经干细胞对损伤后神经细胞的凋亡抑制作用和可能的机制。方法:SPF级SD大鼠20只,以线栓法建立大脑中动脉缺血再灌注模型,随机分为2组,模型组(对照组)10只,干细胞移植组(治疗组)10只。取孕14天的Wistar胎鼠脑皮质神经干细胞培养至第三代,于损伤后的24小时立体定向注射植入成年Wistar大鼠脑缺血损伤局部。于移植后48小时处死鼠取脑,免疫组化检测bcl-2蛋白和westernblot法检测STAT3蛋白表达的变化差异。结果:移植入神经干细胞后,实验组局部STAT3蛋白和bcl-2蛋白的表达都比对照组明显的增强,差异具有统计学意义(P<0.01)。结论:移植入的神经干细胞通过上调STAT3蛋白和bcl-2蛋白的表达,发挥局部神经元的抗凋亡作用,减轻局部缺血再灌注损伤,保护神经功能。
Objective： To study the express changes of STAT3 and bcl-2 in the area of ischemia-reperfusion in Wistar rat brain between neural stem cells （NSCs） transplanted group and not. Research the mechanism that how the NSCs decrease apoptosis of neurocytes. Method： 20 SPF degree Wistar rats, randomly divided into 2 groups, each 10 respectively. A middle cerebral artery occlusion model was reproduced with the intraluminal suture method. Culture NSCs that get from cortex of 14 days embryonal Wistar rats to the third gen- eration. Inject it into the area of ischemia-reperfusion. 48hours later, exam the bcl-2 by immunohistochemical staining and STAT3 by Western blot to research the affect of NSCs on reducing the apoptosis of neurocytes. Result The expression of STAT3 and Bcl-2 of transplanted groups were higher than the control groups （P〈0.01）. Conclusion： The NSCs can reduce neuron apoptosis in the area of ischemia-reperfusion by upregulating the expression of STAT3 and promoting the expression of Bcl-2.
出处
《现代生物医学进展》
CAS
2009年第16期3030-3033,F0002,共5页
Progress in Modern Biomedicine
基金
黑龙江省青年科学基金资助(QC08C95)
