乌鲁木齐市维吾尔、汉、哈萨克族人群NA等位基因频率调查分析

The Neutrophils Antigen Gene Allele Frequency of Uygur,Han and Kazak People in Urumqi Region

目的:通过中性粒细胞抗原(NA)等位基因的频率调查,了解乌鲁木齐地区(乌市)健康、无血缘关系的维吾尔族、汉族、哈萨克族人群NA基因的多态性,为NA相关的输血性疾病建立可靠的基因诊断技术。方法:优化并建立了多重PCR-SSP法,对乌鲁木齐市健康、无血缘关系的维吾尔族120名,汉族118名,哈萨克族102名进行中性粒细胞抗原(NA)的NA1和NA2等位基因频率的调查。结果:维吾尔族NA基因频率(NA10.383,NA20.617),汉族、哈萨克族NA基因频率(NA1分别为0.555、0.510;NA2基因频率为0.445、0.490)。结论:多重PCR-SSP法分析NA基因等位基因频率结果较为可靠,重复性良好,方法操作简单。

Objective： To study the neutrophils antigen gene allele frequency of Uygur, Han and Kazak nationality people in Urumqi region for gene diagnosis of some transfusion diseases. Methods： A optimum multiprimered PCR-SSP（polymerase chain reaction with sequence-specific primers） method was established to screen the netrophils antigen （NA） genotype ofDNA samples isolated from blood donors of 120 Uygur, 118 Han and 102 Kazak nationality people in Urumqi region. Results： The allele frequency of NA 1 of, Uygur, Han,Kazak was 0.383, 0.555 and 0.510, the NA2 was 0.617, 0.445 and 0.490 in this sample volume. The statistical figures shoWed that Uygur and Han had polymorphism ofNA gene （P〈0.05）. Uygur and Kazak, Kazak and Han had no polymer-phism ofNA gene （P〉0.05）. Conclusion： The multiprimered PCR-SSP method was confirmed to be reliable and repeatable in our experiments for NA allele typing.