2型糖尿病鼠脑梗死模型的建立及相关指标分析

Establishment of type 2 diabetes meilitus model in rats with intracerebral infarction and analysis of indicators

目的：在具有胰岛素抵抗特征近似人类2型糖尿病（T2DM）的大鼠身上建立脑梗死模型。方法：SD雄性大鼠120只，其中90只用高脂高糖和链脲佐菌素（STZ）制成T2DM模型，另30只为对照组。对照组和T2DM成模大鼠随机分为假手术组（A1组、B1组）和手术组（A2组、B2组）。采用神经功能缺损量表（NSS）评分、TTC染色与HE染色观察神经功能缺损征、脑梗死体积及病理改变。结果：T2DM成模率为72％，血脂指标与对照组相比差异有统计学意义（P〈0．01），且血脂异常表现与临床T2DM患者近似。A2组NSS评分3—4级者占75％，脑梗死体积为（55．23±9．59）mm^3，脑梗死成模率为75％；B2组NSS评分3—4级者占60％，脑梗死体积为（54．33±6．39）mm^3，脑梗死成模率为53％。结论：高脂膳食结合STZ注射能复制出接近人类T2DM的动物模型，在此基础上制成的脑梗死模型稳定性和重复性好，是研究人类糖尿病脑梗死病理过程较为理想的动物模型。

Objective：To establish an animal model similar to the metabolic abnormality of human Type 2 diabetes mellitus（T2DM） with insulin resistance and cerebral infarction （CI）. Methods： SD male rats（n=120） were divided into the model group（T2DM,90） induced by high-fatty diet and injection of streptozotocin（STZ）and the control group（30）. Ninety of whom were fed high-fatty diet and STZ. Controls were randomly divided into A1 （sham-operated, 5） and A2 （operated, 5）and models were B1 （sham-operated,15 ） and B2 （operated,15）. Neurological severity score （NSS） , triphenyl tetrazolium chloride （TTC） staining and HE staining were adopted to observe dynamically the syndrome of neurological severity, the volume of CI and the pathological changes. Results： The successful rate of T2DM execution was 76% whose LDL, TC, TG were different compared with controls （P〈0. 01）and similar to clinical Type 2 diabetes mellitus. In the model A2, the successful rates of execution was 75%,3-4 grade of neurological deficit scoring 75%and the volume of CI（55.23±9.59）mm3 respectively. In the model B2,the successful rate of execution was 53.3% 3-4 grade of neurological deficit scoring 60%and volume of CI （54.33±6.39）mm3 respectively. The sham group A1 and B1 behavior change normal at 24 h. Conclusion： Type 2 diabetes mellitus animal model is successfully reproduced by using the high fatty diet plus STZ injection. CI rat models with ischemia-reperfusion were stable and repeatable and provide a perfect animal model for investigating pharmacology of diabetes mellitus.