脂肪酸图谱分析稻属相关种的多样性

Diversity of Oryza Species or Varieties by Fatty Acid Methyl Esters Analysis

将脂肪酸转化成脂肪酸甲酯后通过气相色谱法(GC)来测定,具有快速、高效特征,被广泛应用于微生物种、亚种或群落间的相似性和多样性分析。采用该技术对来源于亚洲(亚洲栽培稻、普通野生稻、药用野生稻)、非洲(非洲栽培稻、短舌野生稻、斑点野生稻)、澳洲(澳洲野生稻)和美洲(高秆野生稻)的稻属相关种的样品进行分析,结果表明,脂肪酸图谱能够在种及种以下的亚种或品种水平显示各水稻样品或来源于不同地区的种质资源多样性。可以作为水稻品质育种的筛选指标,或示踪水稻种质的来源,为稻米资源的开发提供依据。

Fatty acid methyl esters （FAMEs） detected by gas-chromatograph is a rapid and effective analysis of microbial diversity among the different microbes. The finger-printing of FAMEs can be used to show the differences and the diversity of Oryza species or varieties,which came from the different geography location, such as Asia （O. sativa, O. rufipogon and O. officinalis）, Afirica（O. glaberrima, O. barthii,O, punctata）, Australia （O. australienis） and America （O. alta）. The results indicated that the components and contents of fatty acids of rice seeds can be used to differentiate the species or cultivated varieties in Oryza genus. The distinguished components in FAMEs may help us to understand the origin of wild rice germplasm and to provide materials and guidelines in the improvement of fat-related traits in rice-bred in various eco-regions.