马尾松嫩茎愈伤组织诱导与增殖

Callus Induction and Proliferation of the Young Stem of Pinus massoniana

以马尾松春梢嫩茎为试验材料，研究了外植体大小及处理方式、培养基类型、激素种类和浓度对马尾松嫩茎愈伤组织诱导和增殖的影响，结果表明：取春季嫩茎为材料，外植体大小以小于2mm的材料随机接种，在改良GD培养基中添加0．30、0．50mg／L的NAA和0．50、2．00mg／L的2，4-D，均有利于马尾松嫩茎愈伤组织的诱导和增殖。2个最适诱导和增殖培养基配方是：改良GD＋0．50mg／LNAA＋2．00mg／L2，4-D＋0．50mg／L6-BA＋2．00mg／LKT和改良GD＋0．50mg／LNAA＋0．50mg／L2，4-D＋2．00mg／L6-BA，其中NAA和2，4-D是诱导马尾松嫩茎愈伤组织所必需的植物激素，较高的NAA生长素浓度（O．50mg／L）和适当的2，4-D植物激素浓度（O．50～2．00mg／L）有利于愈伤组织的诱导和增殖。

Effects of the explant size, treatment,medium and hormone on the callus induction and proliferation of the young stem of Pinus massoniana were studied. Results indicated that explants less than 2 mm on the modified GD medium with NAA（0. 30,0. 50 mg/L） and 2,4-D（0. 50,2.00 mg/L） were suitable for callus induction and proliferation. Two better media were modified GD medium with 0.50 mg/L NAA and 2. 00 mg/L 2,4-D and 0. 50 mg/L 6-BA and 2. 00 mg/L KT or modified GD medium with 0. 50 mg/L NAA and 0. 50 mg/L 2,4-D and 2. 00 mg/L 6-BAo NAA and 2,4-D are necessary plant hormone for callus induction. The NAA with higher concentration（0. 50 mg/L） and 2,4-D with appropriate hormones concentration（0. 50 - 2.00 mg/L） are conducive to callus induction and proliferation. These findings provide foundation for further culturing some elite clones of Pinus massoniana.