EMPO-酪氨酸共价链接物的合成与ESR研究

Synthesis and ESR Study of Covalent Connection of EMPO on L-Tyr

L-Tyr-EMPO,a new EMPO analogue bearing an L-tyrosine methyl ether group,was first synthesized by acylation.Various radicals,including O-·2,·OH,·OR,and ·R,have been efficiently detected and characterized via L-Tyr-EMPO.The half-life of the L-Tyr-EMPO superoxide adduct was estimated to be ca.6.5 min.More importantly,the present study demonstrated a new synthetic strategy for covalent conjugation between cyclic-nitrone and amino group in peptides or proteins,by which the site-specifically spin trapping can be performed via antibody linked nitrone in the near future.Furthermore,with the help of the covalent link,the targeting for the areas of interest in which the monitored radical species was sitespecially generated.

L-Tyr-EMPO, a new EMPO analogue bearing an L-tyrosine methyl ether group, was first synthesized by acylation. Various radicals, including O2^-. , ^.OH,^.OR, and ^.R, have been efficiently detected and characterized via L-Tyr-EMPO. The half-life of the L-Tyr-EMPO superoxide adduct was estimated to be ca. 6.5 rain. More importantly, the present study demonstrated a new synthetic strategy for covalent conjugation between cyclic-nitrone and amino group in peptides or proteins, by which the site-specifically spin trapping can be performed via antibody linked nitrone in the near future. Furthermore, with the help of the covalent link, the targeting for the areas of interest in which the monitored radical species was sitespecially generated.