层流手术中人员流动次数与空气含菌量的关系

Relationship between quantum of bacteria in air and the movement of medical staff in laminar flow operation room

目的了解并评价层流手术室中人员流动对空气含茵量的影响。方法采用平板暴露法对20例手术进行术中空气采样，采样时间从层流30min后手术患者入室前开始至手术结束，每隔30min采样计数空气细菌；同时记录各时间段内人员流动次数。结果层流手术室中各时间段平均人员流动次数及空气菌落数的相关系数为0．945，空气细菌含量与人员流动具有相关性，术中人员流动累计达60人次时空气微生物超过国家规定的普通手术室空气静态标准。结论术中人员流动的次数，直接影响层流手术室空气中的细菌含量，层流手术室术中必须严格限制入室人员。

Objective To investigate and evaluate the effect of personnel movement frequency during operation to quantum of bacteria in air in laminar flow operation room. Methods Air sampIes during 20 operation cases were collected by plat slab exposure method. The sampling - time was from starting laminar flow for 30 minutes （ patients came into operation room at this time）to operation finish. During this time,samples were taken from the rooms every 30 minutes and persons＇ movement frequencv were recorded. Results At each nhase.the correlation coeffcient of persons＇ movement freanency during operation and quantum of bacteria in air in laminar flow operation room was 0. 945. There was correlation between quantum of bacteria in air and persons＇ movement. Quantum of bacteria of air would exceed national sanitary standard of normal operation room when persons＇ movement frequency was accumulated counted to 60. Conclusions During operation,persons＇ movement frequency will ,effect quantum of bacteria in air in laminar flow operation room directly ,so we must strictly limit the number of persons＇ moving in or out operation room.