瘦素(leptin)对猪脂肪细胞脂滴相关蛋白和脂肪生成相关酶mRNA表达的影响

Effect of leptin on porcine adipocytes mRNA expression of lipid droplet related protein and adipogenetic related enzyme

以体外培养的猪原代脂肪细胞为研究对象,研究瘦素(leptin)对猪脂肪细胞脂滴相关蛋白(perilipin、ADRP、TIP47)和脂肪生成相关酶(FAS、SCDα、ACCα)mRNA表达的影响.用0、50、100、150nmol·L-1leptin分别处理脂肪细胞3、6 h,油红O染色鉴定,RT-PCR检测.结果表明,当用150 nmol·L-1leptin处理猪脂肪细胞3 h时perilipin mRNA表达显著降低,用50、100 nmol?L-1leptin处理3 h时PPARγ、ACCα、FASmRNA表达降低;当用50、100 nmol·L-1leptin处理猪脂肪细胞6 h时perilipin、SCDα、PPARγ、FAS、ACCα、LXRαmRNA表达降低,50、100、150 nmol·L-1leptin处理显著下调ADRPmRNA的表达,100、150 nmol·L-1leptin处理TIP47mRNA表达升高.以上结果提示,50、100 nmol·L-1leptin可能通过抑制PPARγ和LXRα来抑制perilipin、SCDα、FAS、ACCαmRNA的表达,从而调控猪原代培养脂肪细胞中的脂质代谢,而150 nmol·L-1leptin可能引起leptin的抵抗进而削弱leptin的调控作用.

To investigate the effect of leptin on porcine adipocytes mRNA expression of lipid droplet related protein （perilipin, ADRP, TIP47） and adipogenetic related enzyme （FAS, SCD α, ACC α）, the adipocytes were treated for 3, 6 h with 0, 50,100,150 nmol. L-1 leptin, and then identified with red oil staining and tested by semiquantitative RT-PCR. The result shows that, when the adipocytes was treated with 150 nmol. L-1 leptin for 3 h, the expression of perilipin mRNA was decreased remarkably; treated with 50 and 100 nmol-L-1 leptin for 3 h, the expression of PPAR 7, ACCa, FAS mRNA were decreased （P〈0.05）; when the adipocytes was treated with 50, 100 nmol. L-1 leptin for 6 h, the expression of perilipin, SCD a, PPARγ, FAS, ACC a, LXR a mRNA were decreased; treated with 50, 100, 150 nmol. L -1 leptin for 6 h, the expression of ADRP mRNA was decreased; treated with 100,150 nmol. L-1 leptin for 6 h, the expression of TIP47 mRNA was increased. The findings above suggested that 50,100 nmol · L- 1 leptin may inhibit the expression of perilipin, SCDα, FAS, ACCα mRNA through inhibiting PPARγ and LXR a to regulate lipid metabolism in the porcine adipocytes, and 150 nmol. L -1 leptin may cause leptin resistance, and then reduced the effect of leptin.