摘要
利用大孔吸附树脂分离岩黄连中的总生物碱,采用静态和动态吸附-解吸附方法,以总生物碱和脱氢卡维丁含量为指标,考察了6种大孔吸附树脂对岩黄连总生物碱的吸附能力,其中D101型大孔吸附树脂的比吸附量和比洗脱量均较高。优化后的提取工艺为:上样药液原药材浓度0.2g/ml,吸附流速为2BV/h,解吸附溶剂为60%乙醇(3BV),所得岩黄连提取物中总生物碱含量大于60%。
The total alkaloids were extracted from Corydalis saxicola Bunting by macroporous resin. Six different macroporous resins were used for extraction of the total alkaloids and dehydrocavidine by using static and dynamic adsorption-desorption experiments. The specific adsorption capacity and eluting of D101 macroporous resin were comparatively high. The optimum parameters of the purification process were established as the sample concentration of 0.2 g/ml at the absorption flow rate of 2 BV/h and with 3 BV of 60 % ethanol as the desorption solvent. The contents of total alkaloids in the purified extract were over 60 %.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2009年第10期750-754,共5页
Chinese Journal of Pharmaceuticals
基金
广西科学技术研究与开发计划项目(桂科攻0992003A-7)
广东省教育部产学研结合项目(2006D90504016)
广东省科技厅国际合作项目(2008A050200005)
关键词
岩黄连
大孔吸附树脂
生物碱
脱氢卡维汀
纯化
Corydalis saxicola Bunting
macroporous resin
alkaloids
dehydrocavidine
purification
