芪薏益肝煎对H22荷瘤小鼠抑瘤作用的实验研究

Experimental study of Qiyiyiganjian on H22 cells-bearing Mice

[目的]观察芪薏益肝煎对H22荷瘤小鼠的抑瘤作用,并探讨其作用机制。[方法]小鼠右腋下皮下种瘤制作实体瘤模型,将30只小鼠分为3组:对照组、中药组和5-氟脲嘧啶(5-Fu)组,各10只,用药15 d后取瘤称重计算抑瘤率;采用免疫组化技术检测存活素(Survivin)和细胞周期素(Cyclins)D1蛋白的表达。[结果]中药组对小鼠肝癌细胞的生长抑制率为37.28%,5-Fu组为50.48%。Survivin及Cyclin D1在3组中均呈阳性表达,中药组Sur-vivin和Cyclin D1表达减弱,与对照组比较差异有统计学意义(P<0.05,<0.01)。[结论]芪薏益肝煎对肝癌细胞的增殖有明显的抑制作用,其作用机制与Survivin及Cyclin D1表达有关。

[Objective]To observe the antitumor effect of Qiyiyiganjian（QYYGJ）on H22 tumor cells in mice.[Methods]After injecting H22 hepatoma cells into their right axila, 30 mice were randomly and equally divided into three groups：control group received treatment of normal solution, treatment group treated by QiYiyiganjian and 5-Fu group treated by 5-Ftu Fifteen days later inhibiting rate was accouted, survivin and cyclin D1 were examined by using immunohistochemistry straining method in the tumor tissue. [Results]The inhibiting rate was 37. 28% in hepatoma cells by using QiYiyiganjian and 5-Fu was 50. 48%. The expressions of survivin and cyclin D1 in three groups were positive. The expression of survivin and cyclin D1 decreased in treatment group. The diferences between the treatment group and control group were significant. [Conclusion]QYYGJ is effective for inhibiting hepatoma cells which relate to expressions of survivin and cyclin D1.