蛹虫草液体菌种通气发酵培养及其营养成分分析

Liquid Fermentation with Ventilation of Cordyceps militaris and Its Components Analysis

利用液体通气发酵培养蛹虫草菌种,分析培养所得不同蛹虫草的有效成分。以体积分数2%的接种量,在pH 7.0、20℃下培养96 h即可达到最佳发酵效果;菌种冷藏时间对发酵效果无显著影响;培养基料量占培养容器体积的1/5,添加5%的营养素为最经济是培养基料配方。利用缫丝后的干蚕蛹成功培养出蛹虫草。对不同培养基(大米、鲜蚕蛹、干蚕蛹)培养的蛹虫草营养成分进行了比较研究:干蛹虫草中虫草素的含量和超氧化物歧化酶(SOD)的酶比活力最高;腺苷的含量以鲜蛹虫草最高;大米虫草多糖含量最高,菌丝体中虫草酸含量最高;4个虫草样品中矿物质元素含量都较丰富,而镉、铅、砷重金属元素含量较低,汞元素未检测到。

The target of this study was to establish and optimize high effective liquid fermentation technology of Cordyceps militaris. Cordyceps rnilitaris strain cultured by liquid medium with ventilation, and analysis the active components of Cordyceps rnilitaris cultured from different mediums. The results demonstrated that the optimum fermentation conditions were pH 7.0, 20 ℃ and 96 h. To the best of our knowledge, this is the first report described that the dried pupa could be one of medium to culture the Cordyceps rnilitaris properly. The nutritional components of Cordyceps rnilitaris cultured by different medium （rice, fresh pupa and dried pupa） have been analysed and shown that the highest cordycepin content and superoxide dismutase （SOD） activity can be achieved at the dried pupa, the adenosine level in fresh pupa was the highest, and the highest polysaccharide content was found in rice. The content of D-mannitol in mycelium was higher than that of other mediums. 4 Cordyceps samples were rich in minerals. The content of heavy metal elements, cadmium, lead and arsenic were low, but no mercury was detected.