摘要
L-苯基乙酰基甲醇(L-phenylacetylcarbinol,L-PAC)是合成麻黄碱等药物的重要手性中间体。利用酵母生物催化法合成L-PAC;采用制备型高效制备液相色谱(RP-HPLC)纯化转化产物,获得高纯度的L-PAC(纯度>99%);采用紫外光谱、红外光谱、旋光、核磁共振及高分辨质谱等技术手段对分离产物进行了结构鉴定。
L-phenylacetylcarbinol (L-PAC) is an important chiral intermediate for ephedrine and pseudoephedrine synthesis. In this study, L-PAC could produced from pyruvate and benzaldehyde by Saccharomyces cerevisiae. Then the crude extract of the bio-resultant was purified using preparative reversed-phase high performance liquid chromatography to achieve purified L-PAC. The result from HPLC chromatogram, which equipped with UV detector at the wave length of 283 nm, indicated that the purity of the purified L-PAC was over 99%. The ultra violet absorbance (UV), infrared (IR), specific rotation, nuclear magnetic resonance (NMR) and mass spectrometry (MS) was further used to identify the structure of L-PAC.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2009年第5期716-720,共5页
Journal of Food Science and Biotechnology
关键词
制备型反相高效液相色谱
L-苯基乙酰基甲醇
麻黄碱
伪麻黄碱
Preparative reversed-phase high performance liquid chromatography (Preparative RP- HPLC), L-phenylacetylcarbinol, ephedrine, pseudoephedrine