流式细胞术检测精索静脉曲张患者精子线粒体膜电位的研究

Evaluation of Sperm Mitochondrial Membrane Potential from Varicocele Patients Using by Flow Cytometry

目的应用流式细胞术检测精索静脉曲张（VC）患者精子线粒体膜电位并探讨其临床意义。方法将67例精索静脉曲张患者分为VC1组（精索静脉曲张1度，n=26），VC2组（精索静脉曲张2度，n=21）和VC3组（精索静脉曲张3度，n=20），以正常生育男性为正常对照组（n=29）。通过计算机辅助精液分析系统进行精液常规分析。精液标本洗涤处理后用荧光染料罗丹明（Rh223）和碘化丙锭（PI）染色后上流式细胞仪分析，检测精子线粒体膜电位（Rh123^＋／PI^-％）。结果VC1，VC2和VC3组精子线粒体膜电位分别为（50．92±17．62）％，（41．34±16．78）％，（26．36±13．61）％，均显著低于正常对照组[（65．12±13．86）％，P〈0．05或P〈0．01]。96例标本中，精子线粒体膜电位与（a＋b）级精子百分率呈显著正相关（r=0．647，P=0．000），而与其他参数无显著相关性（P〉0．05）。结论精索静脉曲张可引起精子线粒体膜电位降低，可能是导致男性不育的重要原因之一。

Objective To detect sperm mitochondrial membrane potential （MMP） of varicocele patients by flow cytometry and investigate its clinical significance. Methods Sixty-seven varicocele patients were divided into VC1 group （grade 1,n = 26）,VC2 group （grade 2,n=21） and VC3 group （grade 3,n=20）. Normal fertile volunteers were selected as control group （n= 29）. Conventional semen analysis was performed by computer-assisted semen analysis （CASA）. Semen samples were washed and then Rh123/PI staining were carried out to evaluate sperm MMP （Rh123^＋/PI^- %） by flow cytometry. Results The sperm MMP of VC1 ,VC2 and VC3 group were（50.92± 17.62）%, （41.34± 16.78）% and （26.36± 13.61 ） % respectively. Lower than that of the control significantly[（65.12± 13.86） % ,P〈0. 05 or P〈0. 01]. There was significant positive correlations between the MMP and the percentage of grade （a＋b） sperm（r=0. 647,P=0. 000） ,but no significant positive correlations with other parameters（P〉0.05）. Conclusion The decreased MMP occurs in the sperm of men with varicoceles,which might be a risk factors of male infertility.