摘要
Objective: The aim of this study was to investigate the relationship between p38MAPK activity and apoptosis during the drug resistance of breast carcinoma cell lines. Methods: Using p38MAPK special inhibitor SB203580 to analyze the effect on the cell apoptosis of MCF-7/ADM cell. Cell apoptosis was analysed by PI staining and flow cytometry (FCM) (F test). 50% inhibition concentration (IC50) of adriamycin on MCF-7/ADM was determined by MTT method (F test) in vitro. MDR-1 mRNA expression was detected by RT-PCR (F test) and Western Blot (F test) respectively. Results: After SB203580 24 h action the MCF-7/ADM’s apoptosis rate was 26.73 ± 4.90%, higher than the control group and untreated group (F = 143.80, P < 0.001). The sensitity to the ADM was improved significantly (F = 148927.1, P < 0.001), and the reversal effect of treat SB203580 group was 68.45%. The P38MAPK protein (F= 685.419, P < 0.001) and MDR-1 mRNA expression after SB203580 24 h action were lower than the control group and untreated group (F = 9139.24, P < 0.001). Conclusion: P38MAPK signal way plays an important role in drug resistance of breast carcinoma cell. p38MAPK can protect MCF-7/ADM cells from apoptosis, and blocking the p38MAPK signal way can increase the apoptosis for breast carcinoma drug resistance cell lines.
Objective： The aim of this study was to investigate the relationship between p38MAPK activity and apoptosis during the drug resistance of breast carcinoma cell lines. Methods： Using p38MAPK special inhibitor SB203580 to analyze the effect on the cell apoptosis of MCF-7/ADM cell. Cell apoptosis was analysed by PI staining and flow cytometry （FCM） （F test）. 50% inhibition concentration （IC50） of adriamycin on MCF-7/ADM was determined by MTT method （F test） in vitro. MDR-1 mRNA expression was detected by RT-PCR （F test） and Western Blot （F test） respectively. Results： After SB203580 24 h action the MCF-7/ADM＇s apoptosis rate was 26.73±4.90%, higher than the control group and untreated group （F = 143.80, P 〈 0.001）. The sensitity to the ADM was improved significantly （F = 148927.1, P 〈 0.001）, and the reversal effect of treat SB203580 group was 68.45%. The P38MAPK protein （F= 685.419, P 〈 0.001） and MDR-1 mRNAexpression after SB203580 24 h action were lower than the control group and untreated group （F = 9139.24, P 〈 0.001）. Conclusion： P38MAPK signal way plays an important role in drug resistance of breast carcinoma ceil. p38MAPK can protect MCF-7/ADM cells from apoptosis, and blocking the p38MAPK signal way can increase the apoptosis for breast carcinoma drug resistance cell lines.
