建立小型猪Ⅰ型糖尿病模型的方法探讨

A Method Probing into Foundation of The Type ⅠDiabetic Models of Minipigs

将５％四氧嘧啶（ａｌｏｘａｎ）按２００ｍｇ／ｋｇ体重经前腔静脉注射入小型猪体内，分别于４、７、１１、１９、２４、３６、４８、７２、９６、１２０ｈ测定小型猪血液生化指标；并于注射四氧嘧啶后７２ｈ进行糖耐量实验，最后做形态学检查。结果表明受试组血糖明显高于对照组，且糖耐量异常。注射四氧嘧啶后的２～３天，受试组血清总蛋白量呈下降趋势；受试组尿糖转阳，而对照组尿糖阴性。病理形态学所见，肾上腺皮质束状区上皮细胞变性，胞浆空泡状，部分区域核浓染；皮质网状区上皮细胞呈空泡状。而两组猪的胰、心肌、肝、脾、肺、胃、肠、主动脉。

e injected 5% alloxan (200mg/kg body weight) into the bodies of minipigs through v.cava cranialis, and determined respectively the blood biochemical indexes of the minipigs at 4、7、11、19、24、36、48、72、96、120hr after the injection; At 72hr, we also carried on glucose endurance test, and at last did the morphological inspect.The result indicated that the measure of blood glucose of the experimented group was obviously higher than that of the controlled group, and its glucose endurance test was abnormal. The measure of the total serum protein of the experimented group presented declining tendency during 2-3 days after the injection. The urine glucose of the experimented group turned positive, and the urine glucose of the controlled group presented negative. On pathological morphology, the epithelial cells in zona fasciculata of the adrenal cortex was denatured, the cell endoplasm presented empty putty state, partly district of the nucleus strongly dyed; The epithelial cell in zona reticularis of the adrenal cortex presented empty puffy state. The other apparatus of all minipigs such as pancreas、myocardium、liver、spleen、lung、stomach intestine、aorta、thyroid gland were not found unusual.