麻体细胞胚胎发生研究初报

PRELIMINARY STUDY ON SOMATIC EMBRYOGENESIS OF RAMIE

将苎麻“浏阳大叶绿”的子叶培养在附加０．５ｍｇ／ＬＣＰＡ、０．０５ｍｇ／ＬＢＲ的ＣＸＷ培养基上，可获得淡黄色或浅灰绿色的颗粒状愈伤组织。将愈伤组织转移至附加０．１ｍｇ／ＬＣＰＡ、０．０５ｍｇ／ＬＮＡＡ、１．５ｍｇ／ＬＺＴ的ＣＸＷ培养基上继代，颗粒状结构非常明显。继续培养在添加２ｍｇ／ＬＭｅｔ，３ｇ／ＬＹＥ的上述继代培养基上，可分化出一些相互独立的胚状体，继而发育成为胚状体幼苗。

The light yellow or grey green granalous calli were induced from ranie cotyledons of “Liuyang Dayelu” on exw medium contatining 0.5 mg/L CPA,0.05 mg/L BR. When subcultured on CXW medium tontaining 0.1 mg/L CPA,0.05 mg/L NAA,1.5 mg/L ZT,the granulous structure became more prominant. After transfered to the same medium adding 2 mg/L Met,3 g/L YE,some somatic embryos were induced and then grew into embryo plantlets. 