摘要
应用基因工程技术,将EGF、GM-CSF基因克隆到pGEM-3Zf(+)载体的EcoRI,BamHI位点上,再将重组融合基因亚克隆到表达载体pBV220的EcoRI,BamHI位点上,在大肠杆菌DH5α中进行表达,SDS-聚丙烯酰胺凝胶电泳和Westernblot表明EGF-GM-CSF融合蛋白获得表达,并且具有EGF、GM-CSF的免疫学活性.这为进一步研究该融合蛋白的功能和肿瘤治疗提供一种新的基因产品.
By means of genetic engineering technology, human EGF cDNA and GMCSF cDNA were to be cloned into the vector pGEM3Zf(+) at the EcoRI and BamHI sites, subsequently subcloned into the expression vector pBV220 of the EcoRI and BamHI sites; a recombinant fusion protein was expressed successfully in high effect in E.coli and confirmed by SDSPAGE and Westernblot. These results provide an important basis for the tumortherapy.
出处
《生命科学研究》
CAS
CSCD
1998年第2期103-108,共6页
Life Science Research
基金
国家自然科学基金
湖南省科委科研基金
关键词
表皮生长因子
融合蛋白
大肠杆菌
GM-CSF
epidermal growth factor
granulocyte macrophagecolony stimulating factor
fused protein
E.coli