人表皮生长因子(EGF)与单核-巨噬细胞集落刺激因子(GM-CSF)融合蛋白在大肠杆菌中的表达

Expression of Recombinant FusionProtein of Human EGF with GMCSF in E.coli

应用基因工程技术，将ＥＧＦ、ＧＭ－ＣＳＦ基因克隆到ｐＧＥＭ－３Ｚｆ（＋）载体的ＥｃｏＲＩ，ＢａｍＨＩ位点上，再将重组融合基因亚克隆到表达载体ｐＢＶ２２０的ＥｃｏＲＩ，ＢａｍＨＩ位点上，在大肠杆菌ＤＨ５α中进行表达，ＳＤＳ－聚丙烯酰胺凝胶电泳和Ｗｅｓｔｅｒｎｂｌｏｔ表明ＥＧＦ－ＧＭ－ＣＳＦ融合蛋白获得表达，并且具有ＥＧＦ、ＧＭ－ＣＳＦ的免疫学活性．这为进一步研究该融合蛋白的功能和肿瘤治疗提供一种新的基因产品．

By means of genetic engineering technology, human EGF cDNA and GMCSF cDNA were to be cloned into the vector pGEM3Zf(+) at the EcoRI and BamHI sites, subsequently subcloned into the expression vector pBV220 of the EcoRI and BamHI sites; a recombinant fusion protein was expressed successfully in high effect in E.coli and confirmed by SDSPAGE and Westernblot. These results provide an important basis for the tumortherapy.