凝固酶阴性葡萄球菌分布及耐药性研究

THE STUDY ON DISTRIBUTION AND DRUG RESISTANCE OF COAGULASE NEGATIVE STAPHYLOCOCCI

本文对１３６株ＣＮＳ进行ＡＰＩ分类，ＰＣＲ检测ＭＲＣＮＳ及纸片法药敏测定，结果表明ＣＮＳ感染以表皮葡萄球菌（占６１７６％）、溶血葡萄球菌（占２５０％）为主。ＭＲＣＮＳ已成为院内感染重要病原菌，占ＣＮＳ的７８７％。纸片法药敏结果显示ＣＮＳ对青霉素类、喹诺酮类、大环内酯类、磷霉素、氯霉素耐药较高，约为４０～７０％，有些高达８０～９０％，而对利福平、米诺环素、阿米卡星、奈替米星及某些头孢菌素类耐药率较低，对万古霉素、去甲万古霉素全部敏感，且ＭＲＣＮＳ的耐药率显著高于ＭＳＣＮＳ（ｐ＜０００１）。

After identifing 136 strains of CNS by using API,we ditected MRCNS by using PCR to amplify mecA gene and detceted the susceptibility of these CNS to 24 kinds of antibacterial drugs by disk diffusion method. The results showed that the main species of CNS were Staphylococci epidermidis (61 7%) and Staphylococci haemolyticus (25 0%). MRCNS have been the important pathogens of noscomial infections and accounted for 78 7% of CNS. The results of disk diffusion method showed that CNS had higher drug resistant rates to penicillinG,quinolones,macrolides,phosphonomycin,chliranpheniocol and lower ones to rifampin,minocyline,aminoglycosides and some cephalosporins.All strains were sensitive to vancomycin and norvancomycin.The drug resistant rates of MRCND were markedly higher than that of MSCNS (p<0 001).