人工复合三倍体鲤与亲本相对DNA含量及倍性分析

RELATIVE DNA CONTENT MEASUREMENT AND PLOIDY ANALYSIS OF ARTIFICIAL MULTIPLE TRIPLOID CARP AND ITS THREE PARENTAL FORMS

本文通过显微荧光光度术测量了人工复合三倍体鲤的红细胞及亲本红鲤、红鲫与散鳞镜鲤个体的红细胞和精子的相对DNA含量。结果表明：每一种亲本的精子DNA含量是其红细胞DNA含量的二分之一，人工复合三倍体鲤DNA含量等于三个亲本精子DNA含量之和，为三个亲本血液红细胞DNA含量之和的二分之一。以外周血淋巴细胞制备染色体标本，人工复合三倍体鲤染色体数为150，其亲本红鲤、红鲫和散鳞镜鲤的二倍体染色体数均为100。研究进一步证明人工复合三倍体鲤与二倍体亲本的染色体倍性和相对DNA含量存在着明显的相关性。

We have used the method of fluorescence microphotometry to compare the relative DNA content of erythrocyte of the artificial multiple triploid fish with DNA contents of erythrocyte and spermatozoa of its three parental forms: red carp, red crucian carp and scatter-scaled mirror carp. The results indicated that the cellular DNA content of the triploid fish is the sum of its three parental spermatozoa DNA contents, and half the sum of the three parental erythrocyte DNA contents.Chromosome preparations made from leucocyte cultures showed that the artificial multiple triploid carp has a chromosomal number of 150, and all the fore parental forms have 100. Therefore, we have proved that there is a close correlation between the artificial multiple triploid carp and its diploid parents in both chromosomal numbers and relative DNA contents.