摘要
激光扫描共聚焦显微镜是近十年发展起来的医学图象分析仪器,现已广泛应用于荧光定量测量、共焦图象分析、三维图象重建、活细胞动力学参数监测和胞间通讯研究等方面。其性能为普通光学显微镜质的飞跃,是电子显微镜的一个补充。本文以美国Meridian公司的ACASULTIMA312为例简要介绍了激光扫描共聚焦显微镜系统的结构、功能和生物学应用前景。
The laser scanning confocal microscopy is a new medical image analysis instrument,which is developed in the last decade.Now it is widely applied in such fields as fluorescent quantitative measurement,confocal image andlysis,3-D reconstruction,kinetic signal monitoring of living cell,cellcell communication researches,etc.In this paper,ACSA ULTIMA 312(Meridian Co.USA) is taken as an example to introduce the principle of confocal microscopy,its functions and biological applications.
出处
《激光生物学报》
CAS
CSCD
1998年第2期131-134,共4页
Acta Laser Biology Sinica
关键词
激光
共聚焦显微镜
粘附细胞
细胞生物学
Laser Confocal Microscopy Adherent Cell Analysis and Sorting(ACAS)