DNA浓度与其Raman光谱强度间的线性相关的分析

The Analysis of the Linear Correlation between RamanIntensity and The Concentration of DNA in Solution

Ｒａｍａｎ光谱法业已广泛应用于生物医学，生物化学和分子生物学等很多领域，尤其是近年来傅立叶转换Ｒａｍａｎ光谱技术（ＦＴ—Ｒａｍａｎｓｐｅｃｔｒｏｓｃｏｐｙ）的兴起，引起人们极大兴趣。本文介绍Ｒａｍａｎ光谱技术应用的一个新领域一对溶液中ＤＮＡ浓度的测定。溶液中ＤＮＡ浓度的测定，是生物化学和生物医学上经常遇到的问题，通常采用紫外分光光度法。当要分析ＤＮＡ构型变化时，最常用的方法是Ｘ线晶体衍射，核磁共振和Ｒａｍａｎ光谱法。为了进一步研究ＤＮＡ构型变化与浓度变化之间的关系，进行了ＤＮＡ的Ｒａｍａｎ光谱强度与浓度间的线性相关研究。以ＨＰＫ１Ａ细胞ＤＮＡ的８１０ｃｍ－１Ｒａｍａｎ光谱峰作为线性相关分析的内标，实验结果表明该ＤＮＡ的Ｒａ－ｍａｎ光谱强度与浓度间存在着良好的线性相关（Ｒ＝０．９９３）。其检出限为０．１０μｇ／μｌ，标准差（ＳＤ）为０．００３（ｎ＝１１），相对标准差（ＲＳＤ）为２．７３％。应用ＦＴ—Ｒａｍａｎ光谱术测定溶液中ＤＮＡ浓度，方法简单，样品不需特殊处理，灵敏和准确。本文详细讨论了该法的操作步骤和注意事项。

This paper presents a mew methodology about the determination of linear correlation between the concentration of DNA and its Raman intensity using by FTRaman spectroscopy.The band at 810cm-1is used as an internal standard.The experimental results show there is a good linear correlation between the Raman intensity and the concentration of DNA(R=0.993).It revealed that the changes of Raman intensity not only indicated the nature of molecular vibration spectrum,but also does molecuar concentration in solution phases.The some skills about the operating on the FTRaman spectroscopy were discussed in detail.Theis is a simple,sensitive method.Its detection limit is 010μg/μl.Its standard deviation is 0.003,relative standard deviation is 2.73%.