从构建的噬菌体抗体库中筛选抗甲肝人单抗

Selection of Human Anti HAV McAb from a Phage Antibody Library

用固相化甲肝抗原，对所构建的噬菌体抗体库进行了３轮淘筛。第３轮淘筛后洗脱下来的噬菌体，较第１轮增加了近１００倍。含有抗体重链基因和轻链基因的重组克隆，也由淘筛前的２５％增至１００％。说明甲肝抗原对抗体库的淘筛，富集了表面呈现甲肝人单抗的噬菌体。经夹心ＥＬＩＳＡ法筛选到抗甲肝病毒噬菌体抗体，并以竞争抑制实验进一步证实了这些抗体的特异性。

The phage displaying antibody fragments were subjected to three rounds of panning with HAV antigen in solid phase.The eluted phage was enriched nearly 100 fold,and the percentage of recombinant clones increased from 25% to 100% after three rounds of panning.The HAV antigen specific monoclonal antibodies were selected by sandwich ELISA,and the specificity of these antibodies was further confirmed by competive inhibition ELISA.