烷基胺玻璃固定化葡萄糖氧化酶测定血糖

Determination of Serum Glucose with Alkylamine Glass Bound Glucose Oxidase

定量分析血糖在门诊和许多疾病如糖尿病,甲状腺机能抗进,粘液腺癌,垂体机能减退,肾上腺机能减退和妨碍葡萄糖吸收等疾病的诊断有重要意义。测定葡萄糖有很多方法,采用葡萄糖氧化酶比色法,由于操作简便,专一性强,灵敏度高,因此比较适合用于常规测定[1]。但是葡萄糖氧化酶的价格高.

Serum glucose was analysed using glucose oxidase from Aspergillus niger immobilized on to alkylamine glass (pore diameter 55 nm)by glutaraldehyde coupling method.The minimum detection limit was 3.6mg/100ml sample.The recovery of added glucose was 95%.A good correlation (r=0.808) was found between glucose values obtained by a standard commercial method and the present method.