摘要
研究了箬叶多糖FⅢ-a及其化学修饰物、亚硒酸钠和GSH对Cu2+诱导的低密度脂蛋白氧化修饰的保护作用.其结果表明箬叶多糖、硫酸酯多糖、硒酸酯多糖可显著抑制脂质过氧化产物(TBARS)及荧光物质的生成,彼此之间无明显差异.但对VE的消耗有着不同的保护作用,其顺序是FⅢ-a>S-FⅢ-a>Se-FⅢ-a,并且具有明显的量效关系.硒或GSH对Cu2+诱导的LDL氧化修饰无明显的抑制,但联合使用在0.125mmol/LNa2SeO3和0.2mmol/LGSH及12.5μmol/LNa2SeO3和0.02mmol/LGSH的浓度下能强烈地抑制TBARS的生成,甚至比正常的LDL还要低.但是对VE的消耗只有较弱的保护作用,硒酸酯多糖与此相似.Na2SeO3在0.125mmol/L时可以明显抑制荧光物质的生成.
The antioxidative activity of Ruoye polysaccharides prepared from Chinese traditional medicine Ruoye (Indocalamus tesselatus),a kind of bamboo leaves and their chemical modified derivatives were studied.Ruoye polysaccharides and their sulfate and selenate could markedly reduce the formation of lipid peroxides and fluorescence products in Cu2+ oxidative modified low density lipoprotein(LDL),but SeFⅢa had a weak inhibitory effect on vitamin E depletion,just like sodium selenite.Sodium selenite and glutathione treated alone did not prevent oxidation,while sodium seleniteglutathione addition with 0.125 mmol/L Na2SeO302 mmol/L GSH or 0.0125 mmol/L Na2SeO3002 mmol/L GSH,could completely inhibit the formation of lipid peroxides.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1998年第4期427-431,共5页
Chinese Journal of Biochemistry and Molecular Biology
关键词
箬叶多糖
硫酸酯多糖
硒酸酯多糖
氧化修饰
Indocalamus tesselatus polysaccharide
Polysaccharide sulfate
Polysaccharide selenate
Low density lipoprotein
Oxidative modification