小地老虎雄蛾中胚层生殖道和附腺的细胞结构和分泌功能

ULTRASTRUCTURE AND SECRETORY FUNCTION OF THE MESODERMAL REPRODUCTIVE TRACT AND ACCESSORY GLANDS IN THE ADULT MALE BLACK CUTWORM, AGROTIS YPSILON

通过光镜、电镜及组织化学等方法，研究了小地老虎生殖前期雄蛾中胚层生殖道和附腺的腺细胞结构和分泌功能，以及与精子形态和数量变化的关系，结果表明：（１）以缢缩位置、解剖形态、细胞结构、分泌方式、精子形态变化和数量变动为依据，将中胚层生殖道划分为修精囊、输精管、贮精囊、精包腺１～５段等８个区段；（２）中胚层生殖道和附腺具有相同的组织层次，自内向外分为单细胞上皮层、底膜、肌肉层和围膜等４层，但缺少表皮质内膜；（３）中胚层生殖道和附腺的腺细胞具有旺盛的合成和分泌蛋白质的能力，主要有内质网型和液泡型两种，前者有发达的粗面内质网和高尔基体，后者具有致密的核糖体和分泌泡；至少有４种分泌方式：即颗粒顶泌、液泡顶泌、胞质局泌和胞间分泌；修精囊、贮精囊、雄性附腺、精包腺１段的顶泌物为糖蛋白性质（ＰＡＳ阳性）、局泌物为非糖蛋白性质（ＰＡＳ阴性）。

Ultrastructure and secretory function of the mesodermal reproductive tract and acessory glands in the adult male black cutworm, Agrotis ypsilon, were studied with light and electron microscopies and histochemical method. The results were summarized as follows: (1) The male mesodermal reproductive tract was divided into eight regions: sperm modification vesicle, vas deferens, seminal vesicle and spermatophore gland in 15 segments, based on the constriction location, anatomical characters, ultrastructure, secretory manner and sperm variation in both form and amount. (2) The histological structure of male mesodermal reproductive tract showed it consisted of four layers: single epithelum, basal lamella, muscle layer and peritoneal membrane, but without a cuticular intima. (3) The secretory cells of the tract and gland had good capacity for protein synthesis and secretion. They were classified into two main types on the basis of organelles in the cytoplasm: (a) endoplasmic reticulum type, which had a lot of rough endoplasmic reticula and Golgi complexes; (b) vacuole type, which had a great number of secretory vacuoles and free ribosomes. There were at least four secretory manners in these cells, i.e. granular apocrine, vacuole apocrine, cytoplasm merocrine and intercellular secretion.