热休克反应对培养的大鼠脑星形胶质细胞的保护作用及对其IL-6分泌的影响

HEAT SHOCK RESPONSE INDUCES ASSISTANCE TO HYDROGEN PEROXIDE AND INCREASES SYNTHESIS OF INTERLEUKIN-6 IN RAT ASTROCYTE IN VITRO

采用MTT还原法和乳酸脱氢酶释放法研究热休克反应对新生大鼠脑星形胶质细胞的保护作用。结果表明，热休克反应能增强星形胶质细胞对H2O2耐受力。实验还测定了热休克反应对新生大鼠脑星形胶质细胞白细胞介素6（IL-6）释放的影响。结果显示，热休克反应后6h，星形胶质细胞IL-6释放明显增多。结果提示，IL－6分泌的增加可能是热休克反应的细胞保护作用机制之一。

Cell viability and cell membrane disruption of rat astrocyte after heat shock response (HSR) were assessed by the analysis of MTT reduction and lactate dehydrogenase (LDH)release. We studied whether HSR would modulate the susceptibility of astrocyte to H2O2induced oxidative stress. Cell viability was assessed by reduction of Mrs. HSR in 43℃water hath for 30 min decreased H2O2 toxicity (P<0.01) to astrocyte. HSR induced decrease in H2O2 (50μmo;/L) toxicity was also shown by the reduction in the release of LDH, which was a marker of cell melnhrane dismption. The result also showed that Prior to the incubation in 43℃ water bath for 30 min strongly increased IL-6 release 6 h (P <0. 05 ) after HSR. The above data suggest that the enhanced release of IL-6 from astrocyte may be one of the mechanisms underlying the cell protective effect induced by HSR.