大同市成年女性HPV感染及基因型分析

Prevalence and type distribution of human papillomavirus infection in women of Datong,China

目的调查山西省大同市成年女性人乳头瘤病毒（HPV）感染及亚型分布情况。方法采用PCR以及线形杂交法（LA）对931名女性宫颈脱落细胞标本进行HPV基因检测和基因分型。结果正常组、低度上皮内损伤（LSIL）组、高度上皮内损伤（HSIL）组及鳞状细胞癌（SCC）组HPV感染率分别为18.6%,60.2%,69.2%和84.6%;高危型HPV感染率明显高于低危型（P〈0.001）;感染高峰为18-25岁年龄组;总体人群中HPV-16、HPV-58、HPV-18、HPV-52以及HPV-33的感染率最高,依次为15.8%,4.7%,3.8%,3.0%和1.8%,而SCC组中,HPV-16、HPV-18、HPV-58、HPV-52以及HPV-33的感染率最高,分别为61.5%,11.5%,7.7%,3.8%,3.8%。结论大同市女性人群HPV感染状况及HPV基因型分布与国内其他地区存在明显差异。

Objective To survey the prevalence and type distribution of human papillomavirus （HPV） infection in women of Datong. Methods A total of 931 cervical specimens from women attending routine Pap smear cytology screening were subjected to FIPV detection and genotyping with PCR-LA assay. Results The overall HPV prevalence was 18.6% in normal specimens,60. 2% in specimens with light epithelial internal lesion,69. 2% in severe epithelial internal lesion, and 84. 6% in specimens with squamous cell carcinoma（SCC） ,respectively. High risk HPV infections were more prevalent than low risk HPV types （ P 〈 0.05 ）. The peak of high risk HPV infection was found in subjects of 18-25 years old. The prevalence of HPV was 15.8 % ,4. 7 % ,3.8 %, and 1.8 % for HPV - 16, - 58,18, and - 52 in all participants, respectively. But In SCC,HPV-16（61.5% ） ,-18（11.5% ） -58（7.7% ） ,-52 （3.8%） and -33（3.8% ） were prevalent types. Conelusion The study demonstrated the geographic variability of oncogenic HPV distribution in Datong, China.