采用Raman光谱研究小麦胚芽8S球蛋白的二级结构

Raman Spectroscopic Study of Secondary Structure of Wheat Germ 8S Globulin

采用显微激光拉曼光谱仪研究了小麦胚芽8S球蛋白的二级结构。结果表明:小麦胚芽8S球蛋白的二级结构主要为β折叠,另外还含有一定量的α螺旋和无规卷曲构象,小麦胚芽8S球蛋白的C—C—S—S—C—C侧链为扭曲—扭曲—扭曲式和扭曲—扭曲—反式两种构型,其酪氨酸残基趋于全部暴露于溶剂中,而其色氨酸残基趋向于埋藏。

Wheat germ 8S globulin is one of the most important components of globulins. Further investigation of wheat germ 8S globulin structure information will be helpful for exploitation and utilization of wheat germ resource. In this study, the secondary structure of wheat germ 8S globulin was determined using Raman spectroscopy. Results indicated that β -sheets followed by a-helical structure were the major component of wheat germ 8S globulin and some random coil structures were also observed in it. C-C-S-S-C-C side chain in this globulin revealed as the transfigurations of gauche-gauche-gauche and gauche-gauche-trans. All tyrosyl residues were exposed completely, while Trp residues were buried inside.