白灵菇多糖的分离纯化及清除自由基研究

Purification and Free Radical Scavenging of Fruit-body Polysaccharide from Pleurotus nebrodensis

对白灵菇子实体粗多糖进行分离纯化与纯度鉴定,并应用化学发光法对多糖清除羟自由基(.OH)和超氧阴离子自由基(O2.)的能力进行研究。结果表明,利用Savag法脱法蛋白质、透析法脱法小分子物质以及醇沉后得到的粗提物,经DEAE-Cellulose52和SephadexG-200柱层析后得到纯品;采用UV、琼脂糖凝胶电泳以及SephadexG-200方法鉴定这种物质PNFPⅡ-a是一种纯多糖;而且这种子实体纯多糖具有较强的抗氧化活性,能显著消除O2.和.OH。

A water-soluble polysaccharide was isolated and purified from fruit-body of Pleurotus nebrodensis through sequential ethanol precipitation by Savag method, DEAE-cellulose 52 and Sephadex G-200 column chromatography to remove proteins and small molecules. This polysaccharide was identified to be PNFP-IIa through UV, agarose gel electrophoresis and Sephadex G-200 column chromatography. Moreover, PNFPII-a revealed high scavenging capability against hydroxyl and superoxide anion free radicals through chemiluminescent assay.