双歧杆菌22-5产胞外多糖发酵条件的优化

Optimizing Fermentation Conditions for High Productivity of Exopolysaccharide from Bifidobacterium 22-5

在5L发酵罐中对双歧杆菌22-5产胞外多糖的发酵条件进行研究。针对发酵液pH值、培养温度和接种量3个主要影响因素,采用L9(34)正交试验对双歧杆菌22-5高产胞外多糖条件进行优化,优化结果为发酵液恒定pH值为7.0,培养温度为31℃,接种量为3.0%。在此优化条件下,胞外多糖的产量达到了5.8g/L,是优化前的1.75倍。

In order to study optimal conditions for high productivity of exopolysaccharide （EPS）, fermentation experiments of Bifidobacterium 22-5 were conducted through orthogonal design based on three factors： pH of fermentation medium, temperature of fermentation medium and inoculation amount. Results indicated that optimal fermentation conditions for high productivity of EPS from Bifidobacterium 22-5 were stable pH at 7.0 for fermentation broth, 31 ℃ as the fermentation temperature and 3.0% inoculation amount. Under these optimal conditions, the productivity of EPS reached 5.8 g/L with 1.75-fold enhancement.