莪术油对肝癌细胞SMMC-7721基因表达的影响

Effects of Turmeric rhizome Naphtha on Gene Expression of Hepatocarcinoma Cell SMMC-7721

目的:检测莪术油对肝癌细胞SMMC-7721生长,及肝癌相关基因表达的影响。方法:利用MTT比色法检测莪术油对肝癌细胞SMMC-7721增殖的影响;利用以肝癌cDNA抑制性消减文库构建的肝癌相关基因微阵列,检测莪术油作用肝癌细胞SMMC-772136h后基因的表达变化。结果:莪术油作用肝癌细胞SMMC-772136h后,细胞生长受到明显抑制,并观察到有23个基因表达下调,3个基因表达上调。结论:莪术油对肝癌肿瘤细胞的作用是通过影响多基因的表达改变实现的。

Objective： To detect effects of Turmeric rhizome naphtha （TRN） on cell growth and gene expression of hepatocarcinoma cell SMMC-7721. Methods： Cell growth of hepatocarcinoma cell SMMC-7721 with TRN treatment was evaluated by MTT assays, and gene expression of hepatocarcinoma cell SMMC-7721 with TRN treatment for 36 hours was explored with microarray of hepatocarcinoma-associated genes, which resulted from suppression subtractive hybridization library. Results： After TRN treatment on hepatocarcinoma cell SMMC-7721 for 36 hours, cell growth was greatly inhibited and gene expression proftling analysis indicated that 23 genes were up-regulated and 3 genes were down-regulated. Conclusion： The effect of TRN treatment on hepatocarcinoma cell SMMC-7721 can be achieved through alteration of multiple genes expression, which provides important clues to understand the molecular mechanism of TRN effects on hepatocarcinoma ceils.