Viili中乳酸菌的分离、鉴定及其产胞外多糖的初步研究

Isolation,Identification and Exopolysaccharide-producing Ability of Lactic Acid Bacteria from Viili

从Viili中分离得到两株具有较高产胞外多糖能力的乳酸菌,其产多糖量可达111.7mg/L和106.5mg/L。对菌株I、菌株II进行16SrDNA序列分析及生理生化鉴定。16SrDNA序列测序和API50CHL试纸条鉴定结果表明:两株菌分别为Lactobacillus paracaseissp.paracasei和Lactobacillus delbrueckii ssp.bulgaricus。

Two exopolysaccharide （EPS）-producing lactic acid bacterial isolates （strains I and II） isolated from viili were identified by 16S rDNA sequence alignment and biochemical and physiological identification with AP150 CHL kits and their EPS-producing abilities were studied. Strains I and II were identified as Lactobacillusparacasei ssp. paracasei and Lactobacillus delbrueckii ssp. bulgaricus, and the EPS yields in skimmed milk fermented by them were 111.7 and 106.5 mg/L, respectively.