丹酚酸B对衰老小鼠学习、记忆的影响及作用机制研究

Effects and Mechanisms of Salvianolic Acid B on Learning Capability and Memory in Aging Mice

目的:研究丹酚酸B的抗衰老作用及作用机制。方法:将30只2月龄小鼠随机分为青年组、模型组及丹酚酸B组,皮下注射D-半乳糖造成衰老模型,同时丹酚酸B组每日腹腔注射丹酚酸B22.5mg/kg,6周后进行跳台和水迷宫实验,并处死小鼠检查脑组织SOD、MDA及线粒体DNA。结果:丹酚酸B组小鼠跳台和水迷宫实验成绩均明显优于模型组,脑组织SOD活力提高、MDA的含量降低,线粒体DNA的量显著降低。结论:丹酚酸B具有抗衰老作用,推测可能是通过抗氧化及影响线粒体DNA而发挥作用的。

Totally 30 mice were randomly divided into young group, aging model group and salvianolic acid B （Sal B） treatment group. Aging model was successfully established by subcutaneous injection of D-galactose. Mice from Sal B treatment group were administered with a dose of 22.5 mg/kg through intraperitoneal injection. Morris water maze test and stand-jumping test were conducted at 6 weeks post-injection. The mice were scarified to determine superoxide dismutase （SOD） activity, malondialdehyde （MDA） and mitochondrial DNA （mtDNA） levels in brain tissue. Sal B treatment group exhibited better performance than aging model group on Morris water maze test and stand-jumping test. Moreover, Sal B treatment significantly reduced cerebral mtDNA and MDA levels and improved cerebral SOD activity in aging mice. Therefore, Sal B has the function to anti-aging due to the promotion of anti-oxidation and reduction of cerebral mtDNA level.