免疫初乳对腹泻大鼠血浆脂肪酸和葡萄糖及肠黏膜sIgA的影响

Effects of Immune Colostrum on Plasma Fatty Acid and Glucose and Intestinal Mucosa sIgA in Diarrhetic Rats

目的:探讨免疫初乳在干预急性感染性腹泻病中对营养代谢的调节作用及其机制。方法:选用32只健康成年SD大鼠,随机分成对照组(C组)、模型组(M组)、普通初乳保护组(NC组)、免疫初乳保护组(IC组)。NC和IC组每天分别灌服普通初乳和免疫初乳(1ml/100gbw),C和M组灌服生理盐水,连续12d。在灌服实验后的5、10d,M、NC和IC组大鼠感染混合的肠侵染性大肠杆菌和沙门氏菌菌液(1×108ml/100gbw)。结果:在实验的第12天,M组和NC组大鼠血浆游离脂肪酸(freefattyacid,FFA)水平分别显著低于和高于对照组,同时M组和NC组大鼠血浆葡萄糖(glucose,Glu)水平分别显著高于对照组,而IC组和对照组无显著差异。此时,NC组大鼠血清血浆胰岛素(insulin,Ins)水显著高于M、IC组。M组和NC组大鼠小肠液免疫球蛋白A(sIgA)水平分别显著高于和低于对照组,而IC组和对照组无显著差异。结论:免疫初乳通过抗大鼠感染性腹泻的保护效应,能够减少肠道病原菌对肠黏膜免疫系统的刺激,调整和维持机体内Glu和FFA含量的相对稳定。

Objectives： To explore regulation effects and mechanisms of immune colostrum on nutrition and metabolism in acute infectious diarrhea diseases. Methods： Thirty-two healthy adult SD rats with half of female and male were randomly divided into a control group （GC）, a model group （GM）, a group protected with normal colostrum （GNC） and a group protected with immune eolostrum （GIC）. Rats in GNC and GIC were orally administered with normal and immune eolostrum at a dose of 1 ml/100 g body weight once a day for 12 days, respectively. Rats in GC and GM were administered saline using the same methods as controls. At 5 and 10 d post-experiment, the rats in GM, GNC and GIC, respectively, were orally infected with mixed enteroinvasive strains containing 1 × 10^8/ml of E. coli and Salmonella typhi at a dose of 1 ml/100g body weight once. Results： Free fatty acid （FFA） level in plasma of GM was obviously lower than that of GC; in contrast, FFA level in plasma of GNC was significantly higher than that of GC. Meanwhile, glucose in plasma of both GM and GNC was obviously higher than that of GC. However, no significant difference was observed between GIC and GC. At the same time, a significantly elevated insulin level in serum of GNC was detected compared with GM and GNC. The slgA level exhibited an increase in GM and a reduction in GNC compared with GC, but no obvious difference was revealed between GIC and GC. Conclusion： Immune colostrum may attenuate stimulations from diarrhea pathogen bacteria on intestinal mucosa immune system to regulate and maintain a relative stabilization of Gin or FFA content and effectively protect anti-infectious diarrhea in rats.